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In this chapter, we explain a detailed procedure to separate your lives OmpA-like proteins, which might be accustomed additional progress the biological researches of OmpA-like proteins.The objective for this section is provide a detailed purification protocol for the surface-layer (S-layer) glycoproteins associated with periodontal pathogen Tannerella forsythia. The process requires detergent based solubilization of the microbial S-layer followed by cesium chloride gra

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58 ± 2.97, 0.92 ± 0.50 and 0.72 ± 0.28 AU/ml, respectively (p  less then  0.001). The study showed significant increases in EC activation markers, i.e. MCP-1, sE-selectin, sVCAM-1 and F1 + 2 in SLE AECA(+) compared to SLE AECA(-) and control groups. However, the indicator of vasculitis (PTX3) was significantly lower in SLE AECA(+). Moreover, multivariate analysis of variance showed a positive correlation between AECAs and sE-selectin and sVCAM-1 levels, but not with PTX3. AECAs were involved in the initial stages of vascular damage in