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There were no differences between elite and sub-elite biathletes in percentage shooting accuracy. However, elites needed shorter shooting times than sub-elites. Both groups showed increased range times with increased workload levels in prone and standing positions. Yet, there was no effect on shooting accuracy. Finally, analyses of a subset of data did not show any effect of final fixation duration on shooting accuracy. CONCLUSIONS Physiological fatigue seems to have no impact on shooting accuracy, but rather affects shooting times in ex