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Nonetheless, the analyses of pollen tube development on 112 liquid and 45 solid media disclosed that solid method with 594 mM raffinose, 0.81 mM H3BO3, 2.04 mM CaCl2 at pH5.8 showed highest pollen germination. Partly or full substitution of raffinose by sucrose, maltose, or sorbitol reduced in vitro germination associated with the pollen assuming an increased metabolic performance or anti-oxidant task of raffinose. In vitro pollen germination diverse between 26 outlines (P 60 min. Viability of fresh whea
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