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Optimum HRT of 12 h, DO of 4.0-4.5 mg/L, and reflux-ratio of 41 were gotten, and no sludge-reflux was required. Autotrophic-denitrification and -dephosphorization supported by the Fe/C galvanic cell reactions added 63.1% and 75.3% of TN and TP elimination, respectively. Microbial characterization disclosed the prominence of autotrophic denitrifiers (e.g., Thiobacillus), AOB (e.g., Nitrosomonas), NOB (e.g., Nitrospira), and heterotrophic denitrifiers (
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