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In this paper, we develop a multispecies coalescent approach which does not depend on any presumption in regards to the relationship between separation and migration whenever calculating parameters and it is known as mstree. mstree can be obtained at https//github.com/liujunfengtop/MStree/ and uses some mathematical inequalities among a few elements, which include the types divergence time, the ancestral populace dimensions additionally the range gene woods, to approximate var