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Moreover, the ATPS exhibited satisfactory recyclability and the fractionation approach could be applied to different types/sources of lignin. Consequently, the work indicates that ATPS is a novel and effective way to fractionate lignin and reduce its molecular weight polydispersity and structural heterogeneity in one step.The influence of phenolic compounds on the enzymatic hydrolysis of cellulose was studied in depth using spectrophotometric techniques, adsorption analysis and Scanning Electron Microscopy (SEM). In this paper for the f