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These findings are important for exploring the medical significance of chlamydial colonization in the gut and for understanding the mechanisms of chlamydial pathogenicity in the genital tract.Target dose homogeneity has historically been a priority in radiotherapy treatment planning. However, in an era of more advanced modulated techniques, there is now greater flexibility in shaping dose distributions suggesting that allowing controlled target dose heterogeneity may consequently improve organ at risk (OAR) sparing. This study sought to d