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This combination of TMSD derivatization and HCD/UVPD fragmentation results in diagnostic product ions that permit distinction and relative quantitation of sn-stereoisomers and the localization of double bonds. Applying this strategy to a total lipid extract from a thyroid carcinoma revealed a previously unreported 182/181 motif, elucidating a structural feature unique to the lipid class.Developing an effective and safe technology to control severe bacterial diseases in agriculture has attracted significant attention. Here, ZnO nanosphere