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Dental caries is an acid-related disease. Current anti-caries agents mainly focus on the bacteriostatic effect in a neutral environment and do not target acid-resistant microorganisms related to caries in acidic milieus. To assess the antibacterial activities of bedaquiline against oral pathogens in acidic milieus. , , and were used to prepare the mono-/multiple suspension and biofilm. The MIC and IC of bedaquiline against were determined by the broth microdilution method. Bedaquiline was compared regarding (i) the inhi